However, the T-cell proliferation and cytokine production induced by Mut 2 and rCuc m 2 were similar. By contrast, Mut 2 displayed a substantial reduction in IgE-binding capacity (57%) and positive responses, as determined by BAT (33%) and SPT (50%), when compared to those of rCuc m 2. Mut 1 showed a slight reduction in IgE binding but an allergenic activity that was similar to recombinant Cuc m 2, as measured by basophil activation test (BAT) and skin prick test (SPT). Mutants (Mut 1 and Mut 2) were generated by changing specific residues of the Cuc m 2 epitope to alanine, produced in Escherichia coli, and purified by chromatographic methods. Since this epitope is highly conserved in most pollen profilins, it may contribute to an explanation of cross-reactivity between pollen and food profilins. An IgE conformational epitope has been identified in Cuc m 2, the major allergen and profilin from melon. Speech by Governor Waller on the U.S.Hypoallergenic mutants with reduced IgE-binding capacity but which show a similar T-cell response to the corresponding natural allergen are ideal tools for immunotherapy, for preventing a possible anaphylactic shock. Welcoming remarks by Governor Bowman at "Toward an Inclusive Recovery," a research seminar sponsored by the Board of Governors of the Federal Reserve System Speech. Factors Affecting Reserve Balances - H.4.1įederal Reserve Board announces approval of application by Columbia Banking System, Inc.Industrial Production and Capacity Utilization - G.17.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. Archives
March 2023
Categories |